Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples
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چکیده
منابع مشابه
Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples.
PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal sta...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.8.2017